D-stem mutation in an essential tRNA increases translation speed at the cost of fidelity.
The efficiency with which aminoacyl-tRNA and GTP-bound translation elongation factor EF-Tu recognizes the A-site codon of the ribosome is dependent on codons and tRNA species present in the polypeptide (P) and exit (E) codon sites.To understand how codon context affects the efficiency of codon Teas recognition by tRNA-bound EF-Tu, a genetic system